Cloning and Plasmid Construction of Soybean Peroxidase Gene for Prokaryotic Expression System

doi:10.7506/spkx1002-6300-201005044

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (5): 197-200.doi: 10.7506/spkx1002-6300-201005044

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Cloning and Plasmid Construction of Soybean Peroxidase Gene for Prokaryotic Expression System

ZENG Jia-yu1，YUAN Hong-xia1，LIAO Shi-qi2,*，ZHOU Si-tong1，LIANG Qiong1

(1. College of Life Science, Northwest Normal University, Lanzhou 730070, China；
2. Gansu Province Institute of Medical Sciences, Lanzhou 730050, China)

Received:2009-05-31 Revised:2009-10-30 Online:2010-03-01 Published:2010-12-29
Contact: LIAO Shi-qi2 E-mail:yuanhongxia520@163.com

Abstract

Abstract:

The cloning and plasmid construction of soybean peroxidase (sbp) for prokaryotic expression system will provide more information to study the structure and function of soybean peroxidase by means of molecular biology. Total RNA was extracted from soybean root. Soybean peroxidase gene was obtained by RT-PCR technique and transferred into pPICZα-A vector through restriction endonuclease digestion. The resulting recombinant pPICZα-A-sbp expression vector was transformed into E. coli and the amplified recombinant pPICZα-A-sbp expression vector was selected and sequenced. Results indicated that cloned DNA sequence from soybean root exhibited 92% homology to the reported sbp (U51191 (GmEpa1) DNA sequence. Therefore, pPICZα-A-sbp was successfully constructed, which will provide base for its expression in Pichia pastoris (Gs115).

Key words: soybean peroxidase, gene cloning, expression vector

CLC Number:

Q785

ZENG Jia-yu1，YUAN Hong-xia1，LIAO Shi-qi2,*，ZHOU Si-tong1，LIANG Qiong1. Cloning and Plasmid Construction of Soybean Peroxidase Gene for Prokaryotic Expression System[J]. FOOD SCIENCE, 2010, 31(5): 197-200.

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Cloning and Plasmid Construction of Soybean Peroxidase Gene for Prokaryotic Expression System

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