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Identification of a Bacillus Strain Producing Lipopeptide and Cloning of Genes Related to Lipopeptide

RAN Junjian1,2, XU Jianhong1, HU Xiaodan1, SHI Jianrong1,*   

  1. 1. Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Key Laboratory of Control
    Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture, Jiangsu Center for Genetically Modified
    Organisms Evaluation and Detection, Collaborative Innovation Center for Modern Grain Circulation and Safety, Institute of Food
    Quality Safety and Detection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2. School of Food Science,
    Henan Institute of Science and Technology, Xinxiang 453003, China
  • Online:2016-09-15 Published:2016-09-22

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Abstract:

A strain named 7F1 which showed strong inhibitory effect on Fusarium graminearum was isolated from
rhizosphere soil of healthy wheat. Using its genomic DNA as the polymerase chain reaction (PCR) template, strain 7F1 was
identified as Paenibacillus polymyxa by 16S rDNA and gyrB sequence analysis. The stability of its antagonistic activity
was evaluated and the detection of lipopeptide synthesis-related genes was conducted by using specific primers. The results
showed that the antagonist produced by strain 7F1 had good heat stability, which could remain stable at 40–90 ℃. The
inhibitory activity was markedly decreased by treatment with proteinase K, pepsin or trysin. The antagonist could exert its
activity in the pH range from 3.0 to 10.0. Using previously reported lipopeptide primers, the lipopeptide synthase genes
bamC, eriSa and ituC were successfully amplified by PCR from the genomic DNA of strain 7F1. These results may provide
an important reference for further separation and application of lipopeptide.

Key words: lipopeptide, Bacillus, identification, antimicrobial activity, gene cloning

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