Abstract:

With polyhexmethylene maleic rosinate, polyethylene maleic rosinate polymer or its copolymer with acrylic acid as matrix, amylase was immobilized on La(III), Eu(III), Tb(III), Y(III) or Dy(III) coordinates of the matrix. The performance of the immobilized enzyme and the mechanism of immobilization were investigated. High activities were observed for the Y(III) and Dy(III) coordinates of the functional polymers. The optimal temperature and pH were both 60 ℃ and 6.03, respectively. The residual activities after used for 4 cycles were 31.49 and 29.76 mg/g·5 min. In addition, the correlation between the activities of the immobilized enzymes and surface area of the matrix as well as pH was established.

Key words: rosin based functional polymers, enzyme immobilization, amylase, rare earth ion