HPLC Determination of Formononetin in Corn Silk

doi:10.7506/spkx1002-6630-200906039

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (6): 178-179.doi: 10.7506/spkx1002-6630-200906039

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HPLC Determination of Formononetin in Corn Silk

ZHANG Yan-qing，LU Xiao-xiang*，DONG Cai-xia

(College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin Key Laboratory of
Food Biotechnology, Tianjin 300134, China)

Received:2008-04-21 Revised:2008-08-08 Online:2009-03-15 Published:2010-12-29
Contact: LU Xiao-xiang*， E-mail:lxxiang@tjcu.edu.cn

Abstract

Abstract:

Objective: To develop an HPLC method for the determination of formononetin in corn silk. Method: Analysis was carried out by using Hypersil C18 (4. 6 mm ×250 mm, 5 μm) column with CH3OH-0.1% H3PO4 (55:45, V/V) as the mobile phase. The flow rate was 0.8 ml/min and the UV detection wavelength was set at 254 nm. Results: There is good linear relationship between integral value (Y) of peak area and formononetin reference substance concentration (X) in the range of 2.3 to 23μg/ml (r = 0.9991). The average recovery of formononetin is 96.93% with the RSD of 1.30%. Conclusion: The method is convenient and accurate, and can be used for the quality determination of corn silk.

Key words: corn silk, formononetin, HPLC

CLC Number:

TQ244.2

ZHANG Yan-qing，LU Xiao-xiang*，DONG Cai-xia. HPLC Determination of Formononetin in Corn Silk[J]. FOOD SCIENCE, 2009, 30(6): 178-179.

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HPLC Determination of Formononetin in Corn Silk

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