Changes in Freshness and Morphology of Ruditapes philippinarum out of Water as well as Expression of Bax Protein

doi:10.7506/spkx1002-6630-200907047

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (7): 206-209.doi: 10.7506/spkx1002-6630-200907047

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Changes in Freshness and Morphology of Ruditapes philippinarum out of Water as well as Expression of Bax Protein

ZHANG Mei-ping1 ZHANG Xi-chun2,* LIN Wei-jin2 CHEN Fa-he2 HUANG Zhi-yong2

(1.School of Life Science, Shanxi Normal University, Linfen 041004, China
2.College of Bioengineering, Jimei University, Xiamen 361021, China)

Received:2008-05-22 Revised:2008-09-04 Online:2009-04-01 Published:2010-12-29
Contact: ZHANG Xi-chun2,* E-mail:xczhang@jmu.edu.cn

Abstract

Abstract:

The survival condition of astartella(Ruditapes philippinarum)out of water was explored as well as the contribution of apoptosis to this process. Astartella were kept from water for 2 , 6 , 12 and 24 h at 4 ℃ and for 2, 4 and 6 h at 25 ℃. The freshness, morphology and the expression of pro-apoptotic protein Bax were determined by measurement of total volatile basic nitrogen with trace expansion assay, HE staining and immunohistochemical assay. Results showed that the freshness decreases with increasing time being out of water and loosening tissue cells and myofibril fragmentation can be observed as well as the further particles formation. The expression of Bax protein displays the similar pattern at 4 ℃ and 25 ℃. However, the speed of changes at 25 ℃ is faster than that at 4 ℃. All these results suggested that the effect of storage temperature and treatment period of being out of water have similar pattern on the freshness and morphology, as well as the expression of Bax protein in Ruditapes philippinarum.

Key words: Ruditapes philippinarum, apoptosis, immunohistochemical method, Bax protein

CLC Number:

S983

ZHANG Mei-ping1 ZHANG Xi-chun2,* LIN Wei-jin2 CHEN Fa-he2 HUANG Zhi-yong2. Changes in Freshness and Morphology of Ruditapes philippinarum out of Water as well as Expression of Bax Protein[J]. FOOD SCIENCE, 2009, 30(7): 206-209.

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Changes in Freshness and Morphology of Ruditapes philippinarum out of Water as well as Expression of Bax Protein

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