Abstract:

The crude polysaccharides that were extracted from dried samples of Artemisi aintegrifolia with hot water were subjected to consecutive protein removal, decoloration, ethanol precipitation and purification on DEAE cellulose DE-52. Three fractions (PSWP-1, PSWP-2 and PSWP-3) were obtained. And these fractions were further purified on Sephadex G-100 column. The elution profile of each fraction on Sephadex G-100 column presented only a single symmetrical peak. The purity and the molecular weights, the monosaccharide compositions, and the chemical structure of these three fractions were analyzed using HPGPC, HPLC and IR spectrometry method, respectively. Results showed that the molecular weight of PSWP-1 was around 1.1513×105D and was composed of rhamnose and glucose at the molar radio of 1.000:1.703; the molecular weight of PSWP- 2 was nearly 1.2491×105D and the glucose was the only hydrolysis product; and the molecular weight of PSWP-3 was around 1.1663 × 105D and the hydrolysates contained rhamnose and glucose with the molar radio of 1.000:2.189. Infrared spectral analysis showed the three fractions were probably kinds of pyran-polysaccharides jointed by β  glycosidic bonds.

Key words: polysaccharides from Artemisi aintegrifolia, separation and purification, HPLC, IR spectrometry