Abstract:

Naringinase (EC3.2.1.40) with α-L-rhamnosidase and β-D-glucosidase activities is being widely applied in debittering of citrus fruit juice and pharmaceutical industries, and has high research value. α-L-rhamnosidase has been cloned and expressed in Escherichia coli. Recombinant naringinase will provide an economical and convenient source for this enzyme in food and pharmaceutical industries. Therefore, preparation methods, enzymatic properties, enzymatic hydrolysis characterization, immobilization and applications of naringinase in industry are systematically reviewed in this article.

Key words: naringinase, debittering, application, immobilization.