Isolation, Purification and Identification of Polysaccharides from Fomes fomentarius

doi:10.7506/spkx1002-6630-200924015

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (24 ): 80-83.doi: 10.7506/spkx1002-6630-200924015

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Isolation, Purification and Identification of Polysaccharides from Fomes fomentarius

LIU Ju-xiang1，JIA Jian-bo2

1. Department of Biological Engineering, Jiangsu Food Science College, Huai an 223003, China；
2. Department of Biological Engineering, Huaiyin Institute of Technology, Huai an 223003, China

Received:2009-06-13 Online:2009-12-15 Published:2010-12-29
Contact: LIU Ju-xiang1 E-mail:liujx0308@163.com

Abstract

Abstract:

The crude polysaccharides were prepared by ethanol precipitation method from the ferment liquid of fomes fomentarius. The polysaccharides were further purified with ion exchange chromatography and gel chromatography. Apart from this, the structure of polysaccharide was analyzed as well as its composition. The result showed that Fomes fomentarius polysaccharide displayed high homogeneity and was comprised of single polysaccharide, which was formed through the polymerization of galactose. The structure was analyzed by IR suggesting that the polysaccharide was in the form of pyranose containing α-glycosidic bond and C=O group.

Key words: Fomes fomentarius, polysaccharide, separation, purification, structural identification

CLC Number:

TS201.23

LIU Ju-xiang1，JIA Jian-bo2. Isolation, Purification and Identification of Polysaccharides from Fomes fomentarius[J]. FOOD SCIENCE, 2009, 30(24 ): 80-83.

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Isolation, Purification and Identification of Polysaccharides from Fomes fomentarius

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