Abstract:

Melamine in milk products was extracted by acetonitrile - trichloroacetic acid solution, and purified by mixed positive ion exchange column, followed by HPLC determination. Melamine was separated by a DBS-C8 column (4.6×150 mm, 5 μm, Thermo) using acetonitrile–cushion solution (1-pentan esulfonic acid sodium salt – citric acid monohydrate) as mobile phase with the flow rate at 1.0 ml/min, and detected at 240 nm. The limit of detection was 0.15μg/ml with the average recovery between 86.39% and 102.60%.This method has been notarized by national authentication committee.

Key words: melamine, HPLC, milk product