Abstract:

Objective: The antioxidant effects of polysaccharides from Coprinus comatus fruit bodies (CCP) were evaluated in the blood and liver of normal and alloxan-induced diabetic mice. Methods: Mice were randomly divided into normal control group and diabetic model groups. The diabetic mice were induced by intraperitoneal injection of alloxan. The mice in diabetic model groups were administered glibenclamide at 100 mg/(kg·d) dosage or CCP at 200 and 400 mg/(kg·d), respectively. After treatment for 30 consecutive days, all of the mice were sacrificed to assay antioxidant parameters in blood and liver. CAT and SOD Isozymes in liver were analyzed by 4%－35% gradient polyacrylamide gel electrophoresis. Results: CCP treatment for 30 consecutive days significantly increased the activities of SOD and GPx in the blood, the activities of total SOD, CuZnSOD, CAT and GPx in the liver of diabetic mice and the levels of GSH and TAOC in both tissues and significantly reduced the contents of MDA. One band for CAT and four bands for SOD were detected in mouse liver and the staining intensities of CAT and CuZnSOD bands were both lower in alloxan-induced diabetic mice and increased to the normal level after CCP treatments; however, the staining intensity of MnSOD bands was higher in alloxan-induced diabetic mice and recovered to the normal level after CCP treatments. No significant differences in the antioxidant parameters in normal mice were observed before and after CCP treatments. Conclusion: CCP has an obvious improving effect on enzymatic and non-enzymatic antioxidant activities and is able to regulate gene expression of antioxidant isozymes. Therefore, CCP exerts an excellent antioxidant activity in the blood and liver of alloxan-induced diabetic mice without antioxidant parameter changes.

Key words: Coprinus comatus, polysaccharide, antioxidation, diabetes, antioxidant enzyme, isozyme