Growth Inhibition Effect of Bog Bilberry Anthocyanin Extract on 3T3-L1 Preadipocytes

doi:10.7506/spkx1002-6630-201015054

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (15): 248-252.doi: 10.7506/spkx1002-6630-201015054

• Nutrition & Hygiene • Previous Articles Next Articles

Growth Inhibition Effect of Bog Bilberry Anthocyanin Extract on 3T3-L1 Preadipocytes

LIU Jia1，David G. Popovich2，JING Hao1,*

1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China；
2. Department of Chemistry, National University of Singapore, Singapore 117543, Singapore

Received:2010-01-08 Online:2010-08-15 Published:2010-12-29
Contact: JING Hao E-mail:haojing@cau.edu.cn

Abstract

Abstract:

Objective: To study the inhibition effect and its mechanism of bog bilberry (Vaccinium uliginosum L.) anthocyanin extract (BBAE) on the growth of 3T3-L1 preadipocytes. Methods: MTT assay, LDH (lactate dehydrogenase) assay, flow cytometry and fluorescence microscope were used for measuring cell growth inhibition (IC50), membrane permeability, cell cycle and the morphological features of apoptotic cells, respectively. Results: BBAE effectively inhibited the growth of 3T3-L1 preadipocytes in a dosage-dependent way, with an IC50 of approximately 214μg/mL. 3T3-L1 preadipocytes showed a significant (P＜0.05) lower LDH release (67.8%) after exposure to BBAE for 72 h than for 24h (89.0%) and 48 h (85.2%). No obvious improvement in the membrane permeability in 3T3-L1 preadipocytes was observed after BBAE treatment. BBAE effectively induced apoptosis of 3T3-L1 preadipocytes, significantly (P＜0.05) increased cell population in the sub-G1 phase, had no significant effect in the G2/M phase and exhibited a block in the S and G0/G1 phases. After incubation for 72 h in the presence of BBAE, 3T3-L1 preadipocytes exhibited typical apoptosis features observed under fluorescence microscope.

Key words: bog bilberry anthocyanin extract, 3T3-L1 preadipocytes, growth inhibition, apoptosis

CLC Number:

R282.71

LIU Jia1，David G. Popovich2，JING Hao1,*. Growth Inhibition Effect of Bog Bilberry Anthocyanin Extract on 3T3-L1 Preadipocytes[J]. FOOD SCIENCE, 2010, 31(15): 248-252.

[1]	LIU Mengdie, JIANG Huiwen, LI Jie, YAN Shoulei, WANG Qingzhang. Apoptotic Process of Fusarium oxysporum Spores Induced by Air Discharge [J]. FOOD SCIENCE, 2021, 42(9): 22-30.
[2]	XUE Hongkun, TAN Jiaqi, LI Qian, TANG Jintian. Procyanidin B2-Induced Apoptosis in MCF-7 Cells and Its Mechanism [J]. FOOD SCIENCE, 2021, 42(9): 91-99.
[3]	YANG Chenglin, HUANG Chao, LIU Juan, HUANG Weimei, YUAN Zhihang, YI Jin’e, LIANG Zeng’enni, WU Jing. Protective Effect of Betulinic Acid on Liver Injury Induced by T-2 Toxin in Mice [J]. FOOD SCIENCE, 2021, 42(9): 107-114.
[4]	GAN Yufei, XUE Zhenglian, ZHOU Jie, WANG Fang, WANG Zhou, LIU Yan. Inhibition and Mechanism of Action of Truncated Phospholiase A1 Accessory Protein S from Serratia marcescens against Escherichia coli [J]. FOOD SCIENCE, 2021, 42(7): 45-51.
[5]	HE Pingping, ZHENG Yajun, ZHENG Mingjing, JIANG Zedong, DU Xiping, ZHU Yanbing, NI Hui, LI Qingbiao. Protective Effect of Bangia fusco-purpurea Polysaccharide on H2O2-Induced Oxidative Damage in Caco-2 Cells [J]. FOOD SCIENCE, 2021, 42(17): 113-120.
[6]	YA Fuli, XIN Yu, ZHANG Chunmei, CHEN Binlin, LI Weiqi, MA Yongjie. Inhibition Effects and Mechanisms of Curcumin on Hydrogen Peroxide-Induced Platelet Apoptosis [J]. FOOD SCIENCE, 2021, 42(13): 151-157.
[7]	WANG Xinyi, ZHANG Yimin, LIANG Rongrong, DONG Pengcheng, LUO Xin, MAO Yanwei. Advances in Understanding the Regulation of Meat Tenderness by Peroxiredoxin 6 [J]. FOOD SCIENCE, 2021, 42(11): 236-243.
[8]	DU Yuemei, HAN Hedan, GUO Zhuoyu, GAO Liping. Effect of Grape Seed Proanthocyanidin Extract on Cisplatin-Induced Apoptosis of Mouse Testicular Sertoli TM4 Cells [J]. FOOD SCIENCE, 2020, 41(9): 98-104.
[9]	HAO Shuai, LI Shuang, WANG Jing, WU Tingting, ZHANG Jiawen, WANG Chengtao. RNA-seq Transcriptomic Analysis of Phycocyanin in Regulating the Inhibition of Non-small Cell Lung Cancer Cells [J]. FOOD SCIENCE, 2020, 41(5): 93-101.
[10]	GUO Yazhou, YANG Xiaoming, LI Yueying. Alkylresorcinols from Wheat Bran Induced Autophagy and Apoptosis of HepG2 Cells [J]. FOOD SCIENCE, 2020, 41(5): 102-107.
[11]	MA Ting, XIA Guanghua, LI Chuan, SHEN Xuanri. Inhibitory Effect of Tilapia Head Phospholipid on RANKL-Induced RAW264.7 Cell Differentiation and Mechanism of Osteoclast Apoptosis Induced by It [J]. FOOD SCIENCE, 2020, 41(3): 144-150.
[12]	TIAN Yan, DENG Fangming, ZHAO Lingyan, LIAO An, LAI Dengni, CHEN Qiujia. Inhibitory Effect of Glucosinolates from Brassica juncea var. integlifolia on HCT116 Human Colon Cancer Cells [J]. FOOD SCIENCE, 2020, 41(23): 172-180.
[13]	HAN Hedan, HAN Zhaoyu, DU Yuemei, LIU Yunfan, LI Ailin, GAO Liping. Effects of Lycium barbarum Fruit Polysaccharides Combined with Cisplatin on Oxidative Damage and Apoptosis in Human Lung Adenocarcinoma Cell Line A549 [J]. FOOD SCIENCE, 2020, 41(15): 194-200.
[14]	YUN Shaojun, CHU Dongyang, HE Xingshuai, ZHANG Wenfang, FENG Cuiping. Effects of Grape Seed Proanthocyanidins on Renal Iron Content, Oxidative Stress and Fas and Bax Gene Expression in Rats with Iron Overload [J]. FOOD SCIENCE, 2020, 41(13): 106-111.
[15]	HOU Ying, MA Wenrui, CHENG Yawen, ZHENG Haoran, TAN Zhilei, JIA Shiru. Apoptosis of Saccharomyces cerevisiae Induced by ε-Poly-L-lysine Combined with Reactive Oxygen Species [J]. FOOD SCIENCE, 2020, 41(10): 81-87.

Growth Inhibition Effect of Bog Bilberry Anthocyanin Extract on 3T3-L1 Preadipocytes

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments