Abstract:

The preparation of high fructose syrup from broken rice based on glucose isomerization to fructose and calcium type ion exchange resin separation between fructose and glucose was optimized using single factor and orthogonal array design methods. The optimal isomerization conditions were found to be: glucose isomerase amount 9 mg/g broken rice; reaction temperature 70 ℃; pH 6.5; and reaction duration 35 h, and the optimal conditions for the separation between fructose and glucose were as follows: separation temperature 70 ℃; sample concentration 20%; elution solvent flow rate 4 mL/min; and sample loading amount 40 mL. The content of fructose of the syrup obtained under the above conditions was 89.64%.

Key words: broken rice, high fructose syrup, isomerization, fructose