Optimization of Culture Medium for Phenyllactic Acid Production by Lactobacillus paracasei W2

doi:10.7506/spkx1002-6630-201023040

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (23): 174-177.doi: 10.7506/spkx1002-6630-201023040

• Bioengineering • Previous Articles Next Articles

Optimization of Culture Medium for Phenyllactic Acid Production by Lactobacillus paracasei W2

LIU Ge1，WANG Li-mei2,3,*

1. College of Food Engineering, Jilin Agricultural University, Changchun 130118, China；2. Department of Biotechnology and
Food Engineering, Changshu Institute of Technology, Changshu 215500, China ；3. Suzhou Key Laboratory of Food and
Biotechnology, Changshu 215500, China

Received:2010-09-16 Online:2010-12-15 Published:2010-12-29
Contact: WANG Li-mei E-mail:wlmqb@126.com

Abstract

Abstract:

The optimal medium for phenyllactic acid (PLA) production by Lactobacillus paracasei W2 was investigated in order to improve PLA yield. Three most significant components affecting PLA production were selected out of seven components using Plackett-Burman design, namely glucose, Tween-80 and phenylpyruvic acid. Their optimal concentrations were determined by steepest ascent method and response surface methodology (RSM) to be 19.5 g/L, 4 mL/L and 3 g/L, respectively. PLA production reached 801 mg/L under these conditions, 1.4-fold higher than before optimization.

Key words: Lactobacillus paracasei W2, PLA, steepest ascent method, Plackett-Burman design, response surface methodology

CLC Number:

Q939.11

LIU Ge1，WANG Li-mei2,3,*. Optimization of Culture Medium for Phenyllactic Acid Production by Lactobacillus paracasei W2[J]. FOOD SCIENCE, 2010, 31(23): 174-177.

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Optimization of Culture Medium for Phenyllactic Acid Production by Lactobacillus paracasei W2

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