Effect of Resveratrol on Glucose Metabolism in Insulin-resistant 3T3-L1 Adipocytes

doi:10.7506/spkx1002-6630-201023063

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (23): 285-288.doi: 10.7506/spkx1002-6630-201023063

• Bioengineering • Previous Articles Next Articles

Effect of Resveratrol on Glucose Metabolism in Insulin-resistant 3T3-L1 Adipocytes

CHEN Si-fan1，KE Liang-ru2，ZHENG Lin1，SHAN Zhi-ming2，ZHOU Ni-man2，FENG Xiang1,*

1. Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University,
Guangzhou 510089, China ；2. Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510089, Chi

Received:2010-03-23 Revised:2010-10-19 Online:2010-12-15 Published:2010-12-29
Contact: FENG Xiang E-mail:fengx@mail.sysu.edu.cn

Abstract

Abstract:

Objective: To explore the effect and mechanism of resveratrol on glucose metabolism in insulin-resistant 3T3-L1 adipocytes. Methods: Insulin-resistant model was established by the induction of dexamethasone in 3T3-L1 adipocytes. The glucose consumption of insulin-resistant 3T3-L1 adipocytes with and without resveratrol stimulation was determined. The mRNA expression of leptin, adiponectin and resistin was determined by real time PCR, and the expression and phosphorylation of AMPKα were determined by Western blotting. Results: After insulin stimulation with the concentration of 0.01, 0.1μmol/L and 1μmol/L, resveratrol could increase the glucose consumption by 1.3, 1.5 folds and 1.4 folds compared with the control (P < 0.05). Meanwhile, resveratrol could increase mRNA expression of adiponectin and leptin and decrease mRNA expression of resistin. Moreover, resveratrol increased the phosphorylation of AMPKα. Conclusion: Resveratrol can improve glucose metabolism in insulin-resistant 3T3-L1 adipocytes. The molecular mechanism may be the phosphorylation of AMPK α by increasing the expression of adiponectin and leptin and inhibiting the expression of resistin.

Key words: resveratrol, insulin resistance, adiponectin, leptin, resistin, fat cells, AMPK

CLC Number:

Q946.8

CHEN Si-fan1，KE Liang-ru2，ZHENG Lin1，SHAN Zhi-ming2，ZHOU Ni-man2，FENG Xiang1,*. Effect of Resveratrol on Glucose Metabolism in Insulin-resistant 3T3-L1 Adipocytes[J]. FOOD SCIENCE, 2010, 31(23): 285-288.

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Effect of Resveratrol on Glucose Metabolism in Insulin-resistant 3T3-L1 Adipocytes

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