Abstract: Cellulase-free xylanase has been widely applied in the food industry as well as the paper and pulp industry. Wheat straw as one of the most important agricultural by-products can be used to induce cellulase-free xylanase production through fermentation. In the present study, the optimal conditions of wheat straw fermentation by Fusarium solani ZH0101 for producing cellulase-free xylanase were 20 g/L wheat straw, 4 g/L KH2PO4, 1 g/L CH3COONH4, 2 g/L yeast extract, initial pH of 6.0 and fermentation time of 14 days. Under the optimal fermentation conditions, the cellulase-free xylanase activity could reach up to 26.85 U/mL, which exhibited a 30% enhancement compared with the fermentation in original medium.

Key words: Fusarium solani ZH0101, xylanase, submerged fermentation