FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (13): 259-263.doi: 10.7506/spkx1002-6630-201113056
• Bioengineering • Previous Articles Next Articles
XIONG Qi-rong,NIU Rui-jiang,XIE Quan-yuan,XIONG Yong-hua,LAI Wei-hua*
Online:
Published:
Abstract: A novel non-chromatographic ion-exchange adsorption technique was developed to purify polyclonal antibody against Vibrio parahaemolyticus from rabbit serum using carboxylated superparamagnetic nanobeads. pH and NaCl concentration of adsorption and desorption buffers and Tween-20 concentration in washing buffer were studied, and the developed method was compared with caprylic acid-ammonium sulfate precipitation and SPA affinity chromatography. The results showed that the polyclonal antibody could be completely adsorbed by magnetic beads in 0.01 mol/L phosphate buffer (pH 6.0) and desorbed in 0.01 mol/L phosphate buffer (pH 8.0) containing 0.3 mol/L NaCl, and unadsorbed proteins were removed by washing twice with washing buffer containing 0.1% Tween-20. After the purification, the polyclonal IgG purity was 82.6%, the recovery yield 58.0%, and the titer 1/64000. In total, 10 mL of rabbit serum could be treated by 1 g of magnetic beads, and the whole process could be finished within 1 h. The method has obvious advantages compared with caprylic acid-ammonium sulfate precipitation and SPA affinity chromatography, thereby presenting a promising application prospect.
Key words: magnetic beads, ion-exchange adsorption, antibody purification
CLC Number:
R392.33
HE Jun-jun,ZHOU Ru-jin,,TANG Yu-bin,HUNAG Min. Ion-Exchange Purification of Polyclonal Antibody from Rabbit Serum Using Magnetic Beads[J]. FOOD SCIENCE, 2011, 32(13): 259-263.
0 / / Recommend
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.spkx.net.cn/EN/10.7506/spkx1002-6630-201113056
https://www.spkx.net.cn/EN/Y2011/V32/I13/259