Cloning and Bioinformatic Analysis of Exopolysaccharide Biosynthesis Genes from Lactobacillus rhamnosus JAAS8

doi:10.7506/spkx1002-6630-201117061

Abstract

Abstract: The exopolysaccharide (EPS) biosynthetic genes of Lactobacillus rhamnosus JAAS8 were located on the chromosome in a 19.7 kb region consisting of 16 open reading frames. GenBank database searching showed that the predicted protein products of the gene cluster from L. rhamnosus JAAS8 had the highest identity with the EPS biosynthetic genes of Lactobacillus. The central portion of the locus was occupied by six genes (welF, welG, welH, welI, welJ and welE) encoding potential glycosyltransferases. Three genes (rmlA, rmlB and rmlC) involved in the biosynthesis of the sugar nucleotide precursor dTDP-L-rhamnose were identified in the EPS biosynthesis locus. The predicted amino acid sequences from six genes (wzd, wze, wzy, wzx, wzr, and wzb) have the most resemble proteins involved in putative polysaccharide chain length determination, polymerization and export.

Key words: Lactobacillus rhamnosus, exopolysaccharide, gene cluster, biosynthesis

CLC Number:

S182

LI Sheng-yu，ZHAO Yu-juan，ZHANG Xue，DAI Da-hai，ZENG Xian-peng，ZHANG Gui-bin，YANG Zhen-nai. Cloning and Bioinformatic Analysis of Exopolysaccharide Biosynthesis Genes from Lactobacillus rhamnosus JAAS8[J]. FOOD SCIENCE, 2011, 32(17): 292-296.

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Cloning and Bioinformatic Analysis of Exopolysaccharide Biosynthesis Genes from Lactobacillus rhamnosus JAAS8

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