Purification and Monosaccharide Composition Analysis of Tartary Buckwheat Polysaccharides

doi:10.7506/spkx1002-6630-201119007

Abstract

Abstract: Tartary buckwheat polysaccharides (TBPs) were extracted by boiling water, precipitated by alcohol and purified by ion-exchange column chromatography. The purity and molecular weight of TBPs were determined by high performance gel filtration chromatography (HPGFC). Polysaccharide samples were hydrolyzed with sulfuric acid solution, and derivatized with 1-phenyl-3-methyl-5-pyrazolone (PMP). The monosaccharide composition of the hydrolysates obtained was analyzed by RP-HPLC. The results showed that TBPs could be obtained by water extraction and alcohol precipitation after protein removal by Savag method. Three polysaccharides, named as TBP-1, TBP-2 and TBP-3, were obtained by DEAE-cellulose column chromatography with molecular weights of 144544, 445656 and 636795, respectively. Liquid chromatographic analysis based on pre-column derivatization showed that TBP-1 and TBP-2 were composed of glucose and TBP-3 of mannose, rhamnose, glucuronic acid, glucose, galactose and arabinose in a molar ratio of 4.32:2.41:1.00:39.8:9.64:2.02.

Key words: tartary buckwheat, polysaccharide, monosaccharide, derivatization, high performance liquid chromatography (HPLC)

CLC Number:

TS207.3

YAN Jun，SUN Xiao-chun，XIE Zhen-jian，HE Gang，YI Yong，FANG Chen，GOU Xiao-jun. Purification and Monosaccharide Composition Analysis of Tartary Buckwheat Polysaccharides[J]. FOOD SCIENCE, 2011, 32(19): 33-36.

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Purification and Monosaccharide Composition Analysis of Tartary Buckwheat Polysaccharides

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