Optimization of Ethanol Precipitation of Exopolysaccharides Produced by Lactobacillus plantarum 70810 by Response Surface Methodology

doi:10.7506/spkx1002-6630-201119041

Abstract

Abstract: The optimal conditions of ethanol precipitation for exopolysaccharides (EPS) produced by Lactobacillus plantarum 70810 were investigated by one-factor-at-a-time experiments and response surface methodology. A mathematical model was developed to reveal the significant effects of each factor and their interactions on the yield of EPS. The optimal parameters for ethanol precipitation of EPS from Lactobacillus plantarum 70810 were 12.56 h precipitation time, 3.9:1 ethanol/fermentation supernatant ratio (V/V) and 3.54-fold condensation of fermentation supernatant. Under the optimal conditions, the model-predicted and experimental values of EPS yield were 323.11mg/L and 320.17 mg/L, respectively, with a relative error of 0.91%.

Key words: Lactobacillus plantarum, exopolysaccharides, response surface methodology, ethanol precipitation

CLC Number:

TS201.3

FENG Mei-qin，QIU Yuan，ZHANG Qi，Mahinur·MUTUVULLA，CHEN Xiao-hong，DONG Ming-sheng. Optimization of Ethanol Precipitation of Exopolysaccharides Produced by Lactobacillus plantarum 70810 by Response Surface Methodology[J]. FOOD SCIENCE, 2011, 32(19): 188-192.

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Optimization of Ethanol Precipitation of Exopolysaccharides Produced by Lactobacillus plantarum 70810 by Response Surface Methodology

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