FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (23): 266-269.doi: 10.7506/spkx1002-6630-201123053

• Bioengineering • Previous Articles     Next Articles

Optimization of Fermentation Conditions for Thermostable α-Amylase Production by Recombinant Pichia pastoris

QIN Ya-nan1,2,MENG Xian-mei1,*,PAN Feng-guang2,*,SUN Xiao-ming1,LI Feng-xiang1,SHI Yan-zhong1   

  1. (1. Key Laboratory of Oil and Foodstuffs Processing, Jilin Business and Technology College, Changchun 130062, China; 2. College of Quartermaster Technology, Jilin University, Changchun 130062, China)
  • Online:2011-12-15 Published:2011-12-21

Abstract: The methanol-induced expression of thermostableα-amylase in a recombinant Pichia pastoris strain with the thermostableα-amylase gene of Bacillus licheniformis that had been constructed in our previous work was investigated during shake flask cultivation. A 4-variable linear regression design was used to explore the effects of 4 main cultivation conditions such as induction time, methanol amount, pH and inoculation amount on the expressed activity of thermostableα-amylase. A regression model describing the relationship between them was established. It was found that the optimal fermentation conditions were 96 h cultivation at an inoculation amount of 150 mL/100mL and a shake speed of 200 r/min in the presence of 0.5% methanol, resulting in a thermostableα-amylase activity of 217.2 U/mL.

Key words: Pichia pastoris, thermostable α-amylase, fermentation, optimization

CLC Number: