Antioxidant Activity of Polyphenol Extracts from Leaves of E. grandis × E. urophylla Guanglin No. 9

doi:10.7506/spkx1002-6630-201201003

Abstract

Abstract:

Crude ethanol extract from the leaves of E. grandis×E. urophylla Guanglin No. 9 was fractionated by repeated extractions sequentially with solvents of different polarity into petroleum ether-soluble, chloroform-soluble, ethyl acetate-soluble, n-butanol-soluble fractions and residue. Total phenolic contents in the six samples were determined by Folin-Ciocalteu method. Meanwhile, their antioxidant activity was tested by in vitro ABTS, DPPH and FRAP assays. The results indicated that among the samples, the ethyl acetate-soluble fraction showed the highest total phenolic content, 502.67 mg/g, and the strongest ABTS and DPPH scavenging activity and reducing power activity, which were better than those of VC. The IC50 values of the fraction against DPPH and ABTS radicals were 0.097 mg/mL and 0.034 mg/mL, respectively. Moreover, a positive correlation between total phenolic content and antioxidant activity was observed for all the samples. This indicates that polypehnolics are mainly responsible for their antioxidant activity. The crude ethanol extract exhibited a total phenloic content (over 30%) similar to that of its counterpart from tea. This study demonstrates that the crude ethanol extract has a high exploitation potential.

Key words: eucalyptus leaf, polyphenol, antioxidant activity, DPPH, ABTS

CLC Number:

Q946

WANG Jun-liang，XIAO Su-yao，CHEN Yun-jiao，CHEN Xue-xiang，TANG Jie，CAO Yong. Antioxidant Activity of Polyphenol Extracts from Leaves of E. grandis × E. urophylla Guanglin No. 9[J]. FOOD SCIENCE, 2012, 33(1): 20-24.

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