Determination of Coupling Ratios of Four Mycotoxins-Carrier Protein Conjugates by Mass Spectrometry

doi:10.7506/spkx1002-6630-201202048

Abstract

Abstract: Three mycotoxins including fumonisin B1 (FB1), ochrotoxin A (OTA) and deoxynivalenol (DON) were coupled with two carrier proteins such as ovalbumin (OVA) and bovine serum albumin (BSA), respectively. Four small molecule-carrier protein conjugates (FB1-OVA, FB1-BSA, OTA-BSA and DON-OVA) were prepared. The coupling ratios of the conjugates were determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and ultraviolet (UV) absorption spectroscopy, respectively. The coupling ratios of FB1-OVA, FB1-BSA, OTA-BSA and DON-OVA were 5:1, 11:1, 3:1 and 0, respectively as determined by MALDI-TOFMS. However, the coupling ratios of OTA-BSA and DON-OVA determined by UV absorption spectroscopy were 4.4:1 and 3.4:1, respectively. The coupling ratios of FB1-OVA and FB1-BSA could not be determined by UV absorption spectroscopy.

Key words: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, ultraviolet absorption spectroscopy, mycotoxin, carrier protein, conjugate, coupling ratio

CLC Number:

S852.4

WANG Ying, WANG Xi-chun, JIN Fu-yuan, HE Cheng-hua, ZHENG Hao, ZHANG Hai-bin. Determination of Coupling Ratios of Four Mycotoxins-Carrier Protein Conjugates by Mass Spectrometry[J]. FOOD SCIENCE, 2012, 33(2): 219-223.

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Determination of Coupling Ratios of Four Mycotoxins-Carrier Protein Conjugates by Mass Spectrometry

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