Identification and Subtyping of Listeria monocytogenes by MALDI-TOF-MS

doi:10.7506/spkx1002-6630-201203040

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (3): 194-198.doi: 10.7506/spkx1002-6630-201203040

• Bioengineering • Previous Articles Next Articles

Identification and Subtyping of Listeria monocytogenes by MALDI-TOF-MS

WANG Yao，CAO Ji-juan，ZHAO Xin，ZHENG Qiu-yue，WANG Gang，TIAN Zhuo，SHI Yuan-yuan，CAO Yuan-yin

1. Institute of Plant Immunology, College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China； 2. Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China；3. Zhuanghe Entry-Exit Inspection and Quarantine Bureau, Zhuanghe 121013, China；4. Yingkou Entry-Exit Inspection and Quarantine Bureau, Yingkou 115000, China

Online:2012-02-15 Published:2012-02-14

Abstract

Abstract: In order to establish a matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) method for the rapid identification and subtyping of Listeria monocytogenes, 37 Listeria monocytogenes isolates were tested using MALDI-TOF-MS and characteristic protein fingerprints from their spectra were acquired and summarized into standard spectra to construct a database for the identification of Listeria monocytogenes. The method was highly reliable as demonstrated by the results obtained by the method for standard Listeria monocytogenes strains. Based on the identification database constructed, the Listeria monocytogenes isolates were classed into 9 subtypes at the protein level.

Key words: Listeria monocytogenes, matrix-assisted laser desorption ionisation time-of-flight mass spectrometry(MALDI-TOF-MS), identification, subtyping

CLC Number:

Q503

WANG Yao, CAO Ji-juan, ZHAO Xin, ZHENG Qiu-yue, WANG Gang, TIAN Zhuo, SHI Yuan-yuan, CAO Yuan-yin. Identification and Subtyping of Listeria monocytogenes by MALDI-TOF-MS[J]. FOOD SCIENCE, 2012, 33(3): 194-198.

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Identification and Subtyping of Listeria monocytogenes by MALDI-TOF-MS

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