Abstract: On the basis of one-factor-at-a-time experiments, Box-Behnken experimental design and response surface methodology were used to optimize process parameters for the crude polysaccharides extracted from Lycium ruthenicum Murr. wine lees by macroporous resin adsorption. The results indicated that AB-8 resin was the best adsorbent for polysaccharide adsorption among 5 types of macroporous tested. The optimal adsorption conditions, resulting in the highest adsorption rate of 92.87%, were pH 9, sample concentration 1 g/L, sample loading flow rate 1 mL/min, and resin diameter-to-height ratio 1:15. The optimal desoprtion conditions were 0.25 mol/L NaCl solution at pH 9 as desorption solvent at a flow rate of 1 mL/min and a dose of 5 BV. After the pooled eluate was condensed and vacuum dried, a purified product containing 67.13% polysaccharides was obtained.

Key words: Lycium ruthenicum Murr. wine lees, polysaccharide, response surface methodology (RSM), purification