FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (10): 10-15.doi: 10.7506/spkx1002-6630-201210003

• Processing Technology • Previous Articles     Next Articles

Optimization of Enzymatic Extraction for Tea Polyphenols from Sweet Tea by Response Surface Methodology

CHENG Ya-fang1,YANG Yang1,WEN Fu-xiong1,YUAN Jing-quan2,MIAO Jian-hua2,*   

  1. (1. College of Life Science and Technology, Guangxi University, Nanning 530004, China; 2. Guangxi Medicinal Herb Garden, Nanning 530023, China)
  • Online:2012-05-25 Published:2012-05-07

Abstract: The extraction process of polyphenols from sweet tea was explored using compound enzyme-assisted extraction. The compound enzymes were composed of cellulose and pectinase at a mixture ratio of 3:4. Based on response surface methodology, the optimal extraction conditions of enzyme-assisted were enzyme dosage of 0.6%, hydrolysis temperature of 45 ℃, extraction pH of 4.95, extraction time of 47.76 min, and solid-liquid ratio of 1:23.58. The ethanol extraction conditions were ethanol concentration of 40% (V/V), extraction time of 70 min, extraction temperature of 70 ℃ and solid-liquid radio of 1:28. Under the optimal extraction conditions, the yield of tea polyphenols was 133.2 mg/g.

Key words: sweet tea, tea polyphenols, enzyme, response surface methodology

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