Optimization of Polysaccharide Extraction from Yeast by Response Surface Methodology

doi:10.7506/spkx1002-6630-201224019

Abstract

Abstract:

Response surface methodology was used to optimize polysaccharide extraction from yeast to enhance polysaccharide yield. Polysaccharide yield was investigated with respect to high pressure treatment time, ultrasonic power and ultrasonic treatment time. A quadratic regression model was established based on a three-variable, three-level Box-Behnken design. The optimum conditions of high pressure treatment time, ultrasonic power and ultrasonic treatment time were found to be 35 min, 510 W and 26 min, respectively. Under these conditions, the predicted value of polysaccharide yield was 29.82%, whereas the actual value was 29.84%. Thus, the optimized extraction procedure is feasible for practical operation and experimental prediction.

Key words: yeast polysaccharide, response surface methodology, extraction, optimization

CLC Number:

References

[1]XU W T, ZHANG F F, LUO Y B, et al.Antioxidant activity of a water-soluble polysaccharide purified from pteridium aquilinum[J].Carbohydr Res,2009,344(2):217-222

[2]史怀, 刘波, 苏明星, 等.淡紫拟青霉胞外多糖的分离、纯化及结构分析[J].生物工程学报,2010,26(8):1080-1087

[3]WASSER S P.Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides[J].Appl Microbiol Biotechnol,2002,60:258-274

[4]王允祥, 吕凤霞, 陆兆新.杯伞（Clitocybe sp）培养条件Box-Behnken法优化研究[J].. 中国农业科学,2005,38(1):145-150

[5]FREIMUND S, SAUTER M, KAPPELI O, et al.A new non-degrading isolation process for 1,3-β-d-glucan of high purity from baker's yeast Saccharomyces cerevisiae[J].Carbohydr Polym,2003,54(2):159-171

[6]KOGAN G, KOCHER A.Role of yeast cell wall polysaccharides in pig nutrition and health protection[J].Livestock Sci,2007,109(1-3):161-165

[7]Bohn J A, Bemiller J N.(1→3)-β-D-Glucans as biological response modifiers: a review of structure-functional activity relationships[J].Carbohydr Polym,1995,89(1):3-14

[8]CIPAK L, MIADOKOVA E, DINGOVA H, et al.Comparative DNA protectivity and antimutagenicity studies using DNA-topology and Ames assays[J].Toxicol in Vitro,2001,15(6):677-681

[9]KOGAN G, STASKO A, BAUEROVA K, et al.Antioxidant properties of yeast (1→3)-β-D-glucan studied by electron paramagnetic resonance spectroscopy and its activity in the adjuvant arthritis[J].Carbohydr Polym,2005,61(1):18-28

[10]SLAMENOVA D, LABAJ J, KRIZKOVA L, et al.Protective effects of fungal (1→3)-β-D-glucan derivatives against oxidative DNA lesions in V79hamster lung cells[J].Cancer Lett,2003,198(2):153-160

[11]KHALIKOVA T A, ZHANAEVA S Y, KOROLENKO T A, et al.Regulation of activity of cathepsins B, L, and D in murine lymphosarcoma model at a combined treatment with cyclophosphamide and yeast polysaccharide[J].Cancer Lett,2005,223(1):77-83

[12]KOGAN G, PAJITINKA M, BABINCOVA M, et al.Yeast cell wall polysaccharides as antioxidants and antimutagens: can they fight cancer[J].Neoplasma,2008,55(5):387-393

[13]王元秀, 张桂香, 李峰, 等.酵母多糖的提取及其对雏鸡免疫器官发育的影响[J].食品科学,2011,32(2):225-259

[14]孙海翔, 尹卓容, 马美范.高压均质破碎啤酒酵母细胞壁的研究[J].食品工业科技,2002,23(2):66-67

[15]WASE D A J, PATEL Y R.Effect of cell 35 on disintegration by ultrasonics[J].J Chem Technol Biotechnol. B: biotechnol,1985,35(2):165-173

[16]WILLIAMS D L, PRETUS H A, MCNAMEE R B, et al.Development of a water-soluble, sulfated (1→3)-β-d-glucan biological response modifier derived from Saccharomyces cerevisiae[J].Carbohydr Res,1992,235:247-257

[17]WANG Y J, YAO S J, WU T X.Combination of induced autolysis and sodium hypochlorite oxidation for the production of Saccharomyces cerevisiae (1→3)-β-D-glucan[J].World Journal of Microbiology and Biotechnology,2003,19(9):947-952

[18]AMBATI P, AYYANNA C.Optimizing medium constituents and fermentation conditions for citric acid production from palmyra jaggery using response surface method[J].World J Microbiol Biotechnol,2001,17(4):331-335

[19]SILVA E M, ROGEZ H, LARONDELLE Y.Optimization of extractionof phenolics from Inga edulis leaves using response surface methodology[J].Sep Purif Technol,2007,55(3):381-387

[20]ANNADURAI G, SHEEJA R Y.Use of Box-Behnken design of experiments for the adsorption of verofix red using biopolymer[J].Bioprocess Eng,1998,18(6):463-466

[21]OIJKAAS L P, WILKINSON E C, TRAMPER J, et al.Medium optimization for spore production of coniothyrium minitans using statistically-based experimental designs[J].Biotechnol and Bioeng,1999,64(1):92-100

[22]ISMAIL A, SOULTANI S, GHOUL M.Optimizatin of the enzymatic synthesis of butyl glucoside using response surface methodology[J].Biotehnol Prog,1998,14(6):874-878

[23]LI Q H, FU C L.Application of response surface methodology for extraction optimization of germinant pumpkin seeds protein[J].Food Chem,2005,92(4):701-706

[24]刘烺新, 陈宗道, 王光慈.啤酒酵母胞壁多糖提取工艺研究[J].重庆大学学报,1994,17(6):43-48

[25]WALTHER P, MULLE M.Double-layer coating for field-emission cryo-scanning electron microscopy-Present state and applications[J].Scanning,1997,19(5):343-348

[26]MURALIDHAR R V, CHIRUMAMILA R R, MARCHANT R, et al.A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources[J].Biochem Eng J,2001,9(1):17-23

[27]崔庆新, 刘军海, 黄宝旭, 等.响应面分析法优化党参多糖提取工艺[J].药物分析杂志,2011,31(5):816-820

[28]黄国荣, 朱俊东, 糜漫天, 等.啤酒酵母多糖提取工艺的研究[J].第三军医大学学报,2006,28(6):封2-01

[29]吴小刚, 吴周和, 吴传茂.啤酒酵母多糖提取工艺条件的研究[J].饲料工业,2006,27(9):27-29