Detection of Vibrio parahaemolyticus in Foods Using F0F1-ATPase Molecular Motor Biosensor

doi:10.7506/spkx1002-6630-201224047

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (24): 226-229.doi: 10.7506/spkx1002-6630-201224047

Detection of Vibrio parahaemolyticus in Foods Using F0F1-ATPase Molecular Motor Biosensor

ZHANG Jie1，GU De-zhou1，ZHANG Hui-yuan1，LIU Yan1，WANG Qi1，ZHANG Xin1，WANG Pei-rong2，TAO Yu-feng3，FAN Fei1，CHEN Guang-quan1,*，YUE Jia-chang2

1. Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China；2. Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China；3. China National Accreditation Service for Conformity Assessment, Beijing 100062, China

Received:2011-10-20 Revised:2012-11-13 Online:2012-12-25 Published:2012-12-12

Abstract

Abstract: An F0F1-ATPase molecular motor biosensor was used to rapidly detect Vibrio parahaemolyticus in foods. For the construction of a biosensor, a specific toxR probe was connected to the ε subunit of F0F1-ATPase avidin-biotin system using an avidin-biotin system. The detection of Vibrio parahaemolyticus was based on differences in ATP production 30 min after separate conjugation of Vibrio parahaemolyticus and negative control to the constructed biosensor. ATP production was determined by measuring the H+ amount in the environment, which was dependent on the fluorescence intensity of F-DHPE. The optimum concentrations of chro toxR and Vibrio parahaemolyticus DNA for detecting Vibrio parahaemolyticu were found to be 40 ng/mL and 40 ng/mL, respectively. In practical applications, the results obtained by this method were in good agreement with those obtained by traditional detection methods and PCR.

Key words: F0F1-ATPase molecular motor, biosensor, Vibrio parahaemolyticus, rapid detection

CLC Number:

TS207.4

[1]	SHEN Jinling, ZHAO Lina, HAN Wei, XU Zhenjian, JIANG Yuan, YANG Jielin, GUO Dehua, XUE Feng. Antimicrobial Resistance, Virulence Gene and Genetic Characteristics of Vibrio parahaemolyticus Isolates from Aquatic Products Imported to Shanghai [J]. FOOD SCIENCE, 2021, 42(8): 264-269.
[2]	TIAN Jingsheng, LI Dongdong, ZHAO Lingyu, GAO Wenhui. Preparation and Application of Molecularly Imprinted Electrochemical Sensor for Rapid Detection of Quinoxaline Drug Residues [J]. FOOD SCIENCE, 2021, 42(6): 316-324.
[3]	LIU Na, CAO Qiang, XIAO Yushi, HUANG Rong, CHENG Lei, LIU Huan, HAN Gang, LI Jincheng, LI Menglong, WU Lidong. Recent Progress of Sensors Based on Dopamine and Its Derivatives in Food Safety Rapid Detection [J]. FOOD SCIENCE, 2021, 42(5): 332-339.
[4]	WANG Xiaorui, QIU Hongling, WANG Shouli, ZHU Haihua, ZHOU Li, PING Yang, TAN Jing, WANG Yong, WANG Hui. Preparation of Monoclonal Antibodies against Outer Membrane Protein K of Vibrio parahaemolyticus and Development of a Double Antibody Sandwich ELISA [J]. FOOD SCIENCE, 2021, 42(4): 319-325.
[5]	WU Youxue, WU Meijiao, TIAN Yachen, WANG Zheng, GUO Liang, LIU Cheng, FANG Shuiqin, LIU Qing. Progress in the Development of Salmonella Biosensors [J]. FOOD SCIENCE, 2021, 42(3): 339-345.
[6]	HUANG Dongwei, GU Guizhang, HU Kena, GAO Xingjie, ZHANG Jinjie, YANG Wenge, XU Dalun. Detection of Guanine in Fish Flesh by Protein Adsorption onto Macroporous Resin Combined with Surface-Enhanced Raman Spectroscopy [J]. FOOD SCIENCE, 2021, 42(18): 298-305.
[7]	YANG Jianfei, MA Qian, ZUO Yong, HUANG Xueqin, XU Jia, WANG Dingli, ZHOU Yaojin, YANG Keyi. A Spectrophotometric Reagent for the Rapid Detection of Nitrite and Its Application to Pickles [J]. FOOD SCIENCE, 2021, 42(18): 321-328.
[8]	ZHANG Buke, SHI Jun, PAN Yingjie, ZHAO Yong, LIU Haiquan. Growth Heterogeneity of Vibrio parahaemolyticus under Different Oligotrophic Conditions [J]. FOOD SCIENCE, 2021, 42(14): 54-61.
[9]	WANG Zouluqi, LI Lihuang, LI Danyang, AI Chaochao, REN Lei, SUN Benqiang. Construction of Down-conversion Fluorescence-Aptamer Immunochromatographic Strip for Rapid Detection of Aflatoxin B1 [J]. FOOD SCIENCE, 2021, 42(12): 295-301.
[10]	LU Jie, TIAN Jing, LIANG Zhenhua, WANG Jinmei, KANG Wenyi, MA Changyang, LI Changqin. Application of Near Infrared Spectroscopy in the Rapid Detection of Total Sugar Content in Lentinula edodes [J]. FOOD SCIENCE, 2021, 42(12): 189-194.
[11]	Fan Yiling, WANG Shujuan, LI Qiongqiong, HU Ying, SONG Minghui, Qin Feng, Liu Hao, YANG Meicheng. Rapid Detection of Shiga Toxin-producing Escherichia coli by Recombinase Polymerase Amplification Combined with Centrifugal Compact Disc Microfluidic Chip [J]. FOOD SCIENCE, 2021, 42(10): 297-304.
[12]	WANG Changchang, MA Liang, LIU Wei, GUO Ting, TAN Hongxia, ZHANG Yuhao, ZHOU Hongyuan, CHEN Lu, LI Daoliang. Advances in Aptasensors Based on Smart Materials for Rapid Detection of Mycotoxins [J]. FOOD SCIENCE, 2020, 41(3): 305-313.
[13]	WU Yuhan, CHEN Wei. Rapid Detection of Tetracycline and Penicillin in Milk by Lateral Immunochromatography [J]. FOOD SCIENCE, 2020, 41(24): 281-286.
[14]	LIU Ruobing, HAO Yihuan, YANG Xi, JIAO Wenya, WANG Xianghong. A Fluorescence Method Based on AccuBlue and Nucleic Acid Aptamer for Detection of Enrofloxacin in Animal-Derived Foods [J]. FOOD SCIENCE, 2020, 41(24): 310-315.
[15]	XIN Liang, ZHANG Lanwei. Recent Progress in Nucleic Acid-Microfluidic Chips Used for Detection of Foodborne Pathogens: A Review [J]. FOOD SCIENCE, 2020, 41(23): 266-272.

Detection of Vibrio parahaemolyticus in Foods Using F0F1-ATPase Molecular Motor Biosensor

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments