Determination of Paraquat in Edible Fungi by Microwave Extraction-High Performance Liquid Chromatography

doi:10.7506/spkx1002-6630-201306041

Abstract

Abstract: An HPLC method was established for the determination of paraquat residues in edible fungi. Samples were extracted with 10 mL of hydrochloric acid solution by microwave digestion extraction at 120 ℃ for 30 min, cleanedup by strong cation exchange (SCX) solid-phase extraction (SPE) column and eluted by saturated ammonium chloride. HPLC separation was carried out by reverse phase chromatography on Atlantis HILIC silica column with a mobile phase composed of acetonitrile and actyl sulfonate at the ratio of 40:60. Paraquat was detected by ultraviolet detector at 259 nm. The limit of quantification (LOQ) based on 10 times signal-to-noise was 1.0 μg/kg. The recovery rate was in the range of 82.33%–96.99% with the relative standard deviations (RSD) of 1.23%–3.90%. This established method is satisfactory for the determination of paraquat residues in edible fungi.

Key words: paraquat, edible fungi, microwave digestion, solid-phase extraction (SPE), HPLC

CLC Number:

TS207．3

TANG Jun1,2，QI Zhong-hua1，SHI Tao-zhong1,2，WU Xiang-wei1,2，CAO Hai-qun1,2，HUA Ri-mao1,2，TANG feng3，YUE Yong-de3,*. Determination of Paraquat in Edible Fungi by Microwave Extraction-High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2013, 34(6): 188-191.

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Determination of Paraquat in Edible Fungi by Microwave Extraction-High Performance Liquid Chromatography

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