FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (10): 289-293.doi: 10.7506/spkx1002-6630-201310064

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Determination of Aflatoxin M1 in Milk by High Performance Liquid Chromatography Using On-line Solid Phase Extraction

DING Jian，LI Pei-wu，LI Guang-ming，WANG Xiu-pin，MA Fei
  

  1. 1. Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan 430062, China；
    2. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China；
    3. Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China；
    4. Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China；
    5. Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China
  • Received:2012-02-26 Revised:2013-04-07 Online:2013-05-25 Published:2013-05-07
  • Contact: Pei-Wu Li E-mail:peiwuli@oilcrops.cn

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Abstract:

Objective: A novel method was developed for the determination of aflatoxin M1 in milk by HPLC with on-line
solid phase extraction (SPE). Methods: An on-line SPE column was prepared through packing SB-C18 filler in stainless
column. The loading and washing solution were water, transferring solution was acetonitrile-water (20:80, V/V), and
elution solution was acetonitrile-water (15:85, V/V). Results: Under optimized conditions, a linear regression equation
was fitted as follows: Y = 2.52746X－0.067 with a correlation coefficient of 0.99906. The detection of limit (LOD) was
0.04 μg/kg, and the limit of quantification (LOQ) was 0.10 μg/kg. Average recovery rates for samples spiked at various
levels were 98%－105.7% with RSD was 0.79%－2.56% (n = 6). Conclusion: A simple, accurate and credible method has
been successfully developed for rapid determination of aflatoxin M1 in milk.

Key words: on-line soild phase extraction, milk, high performance liquid chromatography, aflatoxin M1