Abstract:

The extraction of krill oil from Euphausia pacifica by subcritical 1,1,1,2-tetrafluoroethane (R134a) was
studied and the extracted oil was then analyzed by GC-MS. The effects of three extraction parameters including pressure,
temperature and dynamic extraction time on the yield of krill oil were explored. The maximum extraction yield of krill oil
was 6.69% at the optimal extraction conditions as follows: 12 MPa, 30 ℃ and 40-min dynamic extraction time. Twenty fatty
acids including EPA and DHA were identified from the oil. The total yield of unsaturated fatty acids was as high as 62.35%,
and the total yield of EPA and DHA was 21.82%.

Key words: subcritical R134a, Euphausia pacifica, fatty acid, gas chromatography-mass spectrometry (GC-MS)