Detection and Identification of Alicyclobacillus by Use of PCR and Automated Ribotyping

doi:10.7506/spkx1002-6630-201316040

Abstract

Abstract:

A rapid detection and identification method for Alicyclobacillus was proposed by combined use of PCR and
automated ribotyping (RiboPrinter). A total of 59 pure strains were isolated and identified by the method. The results
showed that automated ribotyping allowed more rapid, accurate and reliable identification with maximum interference
avoidance, the further classification of identified species and enabled their traceability. The results of this study are
expected to provide useful data to establish a database for Alicyclobacillus typing. However, it remains unclear that
whether ribotyping can indicate regional distribution characteristics of Alicyclobacillus due to limited sources and number
of strains in this study.

Key words: Alicyclobacillus, polymerase chain reaction (PCR), RiboPrinter, ribotyping

ZHANG Yu-xia，WEN Zhao-hui，LI Ru. Detection and Identification of Alicyclobacillus by Use of PCR and Automated Ribotyping[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201316040.

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Detection and Identification of Alicyclobacillus by Use of PCR and Automated Ribotyping

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