Simultaneous Determination of Folic Acid, Vitamin B12 and Biotin in Infant Formula by HPLC-MS-MS

doi:10.7506/spkx1002-6630-201322054

Abstract

Abstract:

A method using high performance liquid chromatography tandem mass spectrometry (HPLC-MS-MS) for
simultaneous determination of folic acid, vitamin B12 and biotin in infant milk power was presented. Samples were
sequentially extracted and then subjected to isoelectric precipitation (pH 1.90/4.70). The analysis was carried out on an
SB-Aq column (2.1 mm × 100 mm, 1.8 μm) with isocratic elution using a mobile phase made up of 0.1% (V/V) aqueous
formic acid and acetonitrile (16.5:83.5, V/V). The analytes were detected in the MRM mode. Folic acid, vitamin B12 and
biotin in samples could be separately well within 5 min. The calibration curves were linear over the concentration range of
3.5 - 250 ng/mL for folic acid, 0.8 - 5 ng/mL for vitamin B12, and 0.7 - 50 ng/mL for biotin. The average recovery rates
were 97.8%, 102.3% and 96.9%, with RSD of 1.33%, 0.9% and 1.37% for folic acid, vitamin B12 and biotin, respectively.
The developed method is precise, accurate, sensitive, fast and simple, which can thereforebe applied for the analysis of folic
acid, vitamin B12 and biotin in infant formula.

Key words: folic acid, vitamin B12, biotin, high performance liquid chromatography tandem mass spectrometry (HPLCMS-MS), isoelectric precipitation

CLC Number:

R151.3

WANG Feng-ling，LIU Ai-guo，SUN Jia-jia，PENG Cheng. Simultaneous Determination of Folic Acid, Vitamin B12 and Biotin in Infant Formula by HPLC-MS-MS[J]. FOOD SCIENCE, 2013, 34(22): 269-272.

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