Genetic Diversity Analysis of Saccharomyces cerevisiae Strains by Microsatellite Marker Technique

doi:10.7506/spkx1002-6630-201401025

Abstract

Abstract:

In order to analyze the genetic diversity of indigenous wine yeasts and the differences between indigenous isolates
and commercial wine yeast strains, genetic variability of 28 isolates from Jingyang, Shaanxi Province and 18 commercial
strains was analyzed using four microsatellite markers (ScAAT1, YOR267C, C11 and YPL009C). Meanwhile, genetic
parameters were also analyzed by PopGen32. Forty-six strains were amplified by four microsatellite prime pairs, and
generated a total of 66 polymorphic bands with 16–18 alleles for each locus. The average polymorphism information content
(PIC) of all strains at four loci was 0.862 3, suggesting that all the four SSR loci were hypervariable. Forty-six strains gave
39 different patterns with a discriminatory power of 98.94%. The heterozygosity was observed as 0.478 3–0.658 5. These
results indicate that indigenous wine yeasts and commercial yeasts have high levels of genetic diversity, and both have
unique alleles. Both kinds of strains have clear clustering boundary in the phylogentic tree.

Key words: microsatellite, commercial yeasts, indigenous wine yeast, genetic diversity

CLC Number:

Q93.331

ZHANG Liu-yan1, HUANG Ying-zi1, LIU Yan-lin1,2,*. Genetic Diversity Analysis of Saccharomyces cerevisiae Strains by Microsatellite Marker Technique[J]. FOOD SCIENCE, 2014, 35(1): 130-133.

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