Simultaneous Determination of 10 Synthetic Pigments in Dried Shrimps Using Solid-Phase Extraction-High Performance Liquid Chromatography

doi:10.7506/spkx1002-6630-201404035

Abstract

Abstract:

A high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of 10
synthetic pigments (new red, lemon yellow, sunset yellow, carminum, azorubin, amaranth, allura red, light blue, erythrosine and
rhodamine B) in dried shrimps. Samples were extracted using aminated ethanol and cleaned up on a solid phase extraction column.
The chromatographic separation was achieved on an ODS-3 C18 column using a mobile phase consisting of methanol and 0.02 mol/L
ammonium acetate by gradient elution. Ten synthetic pigments were detected by a diode array detector at the wavelength of 360
nm and quantified by an external standard method. There was a good linear correlation between the concentrations of 10 synthetic
pigments and their peak areas in the range of 0.2–50 mg/L. The detection limits of 10 synthetic pigments were 0.4–1.0 mg/kg. The
recoveries were in the range from 74.3% to 90.0% and the RSDs were less than 4.3%.

Key words: solid phase extraction, high performance liquid chromatography, dried shrimps, synthetic pigments

CLC Number:

O657.72

LIU Hui-hui1, GONG Xiang-hong1, XU Ying-jiang1, ZOU Rong-jie2, LI Jia-wei2, DENG Xu-xiu2, ZHANG Xiu-zhen1,*. Simultaneous Determination of 10 Synthetic Pigments in Dried Shrimps Using Solid-Phase Extraction-High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2014, 35(4): 170-173.

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