Abstract:

This study reports the optimization of fermentation conditions for the production of bacteriocin by Bacillus subtilis
HJD.A32. using response surface methodology. The optimal fermentation conditions were determined to be fermentation in a
medium at pH 5.3 at 30 ℃ for 30 h with an inoculum density of 4% and an inoculum age of 24 h while shaking at 147 r/min.
The potency of bacteriocin produced under the optimal conditions after being treated or not with catalase was raised
respectively by 100% and 300% as compared to that produced under the original fermentation conditions.

Key words: bacteriocin, fermentation, response surface methodology, optimization