Abstract:

Objective: In this study, the protective effect of fucolysated chondroitin sulfate from the sea cucumber Cucumaria
frondosa (Cf-CHS) on kidney was accessed in high fat diet-(HFD) induced diabetic mice. Methods: To establish diabetic
models, mice were fed with HFD. The diabetic mice were administered with different doses of Cf-CHS daily for 19 weeks.
Urine was collected to analyze the levels of glucose, microalbumin (mAlb), total protein, urea nitrogen (UN), uric acid (UA),
creatinine (Cr) and the excretion of N-acetyl-β-glucosaminidase (NAG). Kidney tissue was collected to make microscope
slides. Results: Cf-CHS could significantly (P ＜ 0.01) decrease the levels of glucose, mAlb, total UN, UA, Cr, and NAG
in urine and observably improve kidney tissue of diabetic mice. Conclusion: Cf-CHS has a remarkably protective effect on
kidney of diabetic mice.

Key words: diabetes mellitus, Cucumaria frondosa, fucolysated chondroitin sulfate(CHS), urine protein, N-acetyl-β-glucosaminidase(NAG), kidney protection