Abstract:

In this study, we prepared seabuckthorn Vp powder from seabuckthorn juice through high-temperature
sterilization and subsequent spray drying. The contents of amino acids and vitamins in the Vp powder were detected. In
order to investigate its immunoenhancing effect in mice, immunity-deficiency mouse models were induced by intraperitoneal
injection of cyclophosphamide. The thymus and spleen indices, routine blood parameters, macrophage phagocytosis,
serum hemolysin level and spleen cell proliferation ability were measured. Results showed that seabuckthorn Vp powder
contained a variety of essential amino acids, and vitamin C content was as high as (18 266.4 ± 201.1) mg/kg. Compared
with the model group, the Vp powder (1.0 and 2.0 g/kg) could significantly enhance thymus and spleen indexes in mice (P ＜ 0.01),
increase the amount of white blood cells, reduce the proportion of lymphocytes (P ＜ 0.05), enhance the phagocytic
index of mononuclear macrophages (P ＜ 0.01) and serum hemolysin level (P ＜ 0.01), and improve spleen lymphocyte
transformation. In conclusion, seabuckthorn Vp powder can obviously enhance the immune function of mice.

Key words: seabuckthorn Vp powder, immune function, cyclophosphamide, mice