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Optimization of Antioxidant Combinations for Algal Oil by Response Surface Methodology

LÜ Junwei1,2, YANG Xianqing1,*, LIN Wanling1, LI Laihao1, MA Haixia1, WEI Ya1   

  1. 1. Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, National Research and Development Center for Aquatic
    Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;
    2. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
  • Online:2015-03-25 Published:2015-03-17
  • Contact: YANG Xianqing

Abstract:

The concentrations of rosemary powder (containing 30% or more carnosic acid), VE and VC added together to
algal oil for improved oxidative stability were optimized using Box-Behnken experimental design combined with response
surface analysis based on peroxide value (POV), thiobarbituric acid (TBA) value and docosahexaenoic acid (DHA) content.
A quadratic regression model was established. The optimal combination of the three antioxidants was 0.37‰ rosemary
spices, 0.11‰ VE, and 0.20‰ VC palmitate. The POV value was 9.26 meq/kg, TBA value 0.35 mg/kg and DHA content
30.27% under the optimized conditions after 12 day of accelerated oven-oxidation test. A good agreement was observed
between the predicted and actual values indicating that the established model in this study is feasible.

Key words: algal oil, antioxidants, response surface methodology

CLC Number: