Abstract:

Totally 13 strains of facultative anaerobic bacteria that can produce amylase were isolated from a highquality
liquor pit mud. One strain was screened by determining the activities of α-amylase, β-amylase, glucoamylase and
debranching enzyme. Through morphological observation, physiological and biochemical tests and molecular biological
methods, the screened strain was identified as Bacillus amyloliquefaciens and named as MSP13. Furthermore, the culture
conditions for amylase production were optimized to be 0.15 g/L CaCl2, 0.3 g/L MgSO4·7H2O, pH 5.5 and 37 ℃. This strain
under the optimal conditions could improve the four enzyme activities by 31.645% on average.

Key words: amylase, liquor pit mud, identification, screening, liquor