Abstract:

In the present study, the effects of grinding time, extraction solvents and different amounts of masking agents
on the detection of zearalenone were explored. The most stable results were obtained when corn samples were ground for
more than 180 s, and then extracted with 70% methanol using phosphate buffer solution with 0.025% fish skin gelatin as
the masking agent. The findings of this study can provide a reference for the development of highly stable ELISA kit for the
detection of zearalenone in corn.

Key words: mycotoxin, zearalenone, enzyme-linked immunosorbent assay, quantitative detection