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Optimization of the Extraction Process for Flavonoids from Lotus (Nelumbo nucifera Gaertn.) Receptacle and Their Antioxidant Activities

HU Weicheng1, WANG Xinfeng1, SHEN Ting1, WANG Yuning2, CHEN Ming1, YOU Long1, JI Lilian1,*, LI Pengxia2,*   

  1. 1. Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Jiangsu Collaborative Innovation Center of Regional
    Modern Agriculture & Environmental Protection, Huaiyin Normal University, School of Life Science, Huaiyin Normal University,
    Huai’an 223300, China; 2. Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Online:2015-12-25 Published:2015-12-24
  • Contact: JI Lilian1, LI Pengxia

Abstract:

Using Box-Behnken design and response surface methodology, the optimized extraction conditions for maximum
yield (83.2 mg rutin equivalents/g) of total flavonoids from lotus (Nelumbo nucifera Gaertn.) receptacle were determined
as follows: temperature, 60 ℃; ethanol concentration, 48%; ratio of liquid to solid, 40:1 (mL/g); and extraction duration,
10 min. The flavonoids extracted from lotus receptacle (FLR) exerted remarkable scavenging effects on 1,1-diphenyl-2-
picrylhydrazyl (DPPH) free radical and exhibited high reducing power. Moreover, FLR pretreatment effectively elevated cell
viability in H2O2-treated human skin fibroblast cells. The results obtained from in vitro models clearly suggest that FLR, as a
byproduct of the agricultural and food industries, is a potential source of natural antioxidant.

Key words: response surface methodology, lotus receptacle, total flavnoids, ultrasound-assisted extraction, free radicals

CLC Number: