Separation, Purification and Structural Characteristics of Polysaccharides from Red Skin of Lotus Seeds

doi:10.7506/spkx1002-6630-201615016

Abstract

Abstract:

The crude polysaccharides isolated from the red skin of lotus seeds by enzymatic extraction and ethanol
precipitation were separated into neutral and acidic polysaccharides by DEAE-C column chromatography and subsequent
Sephadex G-25 gel column chromatography. Gel permeation chromatography (GPC) analysis showed that the molecular
weights of the neutral and acidic polysaccharides were 3.78 × 104 D and 4.94 × 104 D with a purity of 91.16% and 90.24%,
respectively. The neutral polysaccharide was composed of 4 monosaccharides including glucose and xylose, while the
acidic polysaccharide was mainly composed of 5 monosaccharides including glucose, xylose and galactose. Infrared spectra
preliminarily confirmed that both purified fractions were pyranoses with α configuration. This conclusion was further proved
by nuclear magnetic resonance.

Key words: red skin of lotus seeds, polysaccharide, separation and purification, structural characterization

CLC Number:

TS201.2

GAO Hang, GAO Yanfen, XU Hong. Separation, Purification and Structural Characteristics of Polysaccharides from Red Skin of Lotus Seeds[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201615016.

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Separation, Purification and Structural Characteristics of Polysaccharides from Red Skin of Lotus Seeds

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