Effects of Folate Metabolism Inhibitors on the Proliferation and Differentiation of OP9 Mouse Stromal Cells

doi:10.7506/spkx1002-6630-201615036

Abstract

Abstract:

Folate is a water-soluble vitamin B which has important physiological functions. The effects of methotrexate
(MTX), trimethoprim (TMP), and pyrimethamine (PTM) on the proliferation and differentiation of OP9 mouse stromal
cells were investigated by methyl thiazolyl tetrazolium (MTT) assay, cell morphology analysis and induced differentiation
combined with oil red O staining. MTX, TMP and PTM significantly inhibited the proliferation of OP9 cells in a dosedependent
manner within a certain concentration range. Cell morphology analysis showed that MTX, TMP and PTM were
cytotoxic to OP9 cells at high concentration. The results of inverted electron microscopic observation and oil red staining
showed that only TMP significantly inhibited the differentiation of OP9 cells in a dose-dependent manner in the range of
500–1 000 μmol/L. The transcription levels of DHFR and MTHFD1 were down-regulated by TMP, which may explain why
the differentiation of OP9 cells was inhibited by TMP.

Key words: dihydrololate reductase inhibitors, OP9 mouse stromal cells, lipid synthesis, proliferation, differentiation

CLC Number:

WANG Hongchao, LIU Yuan, GU Zhennan, CHEN Haiqin, ZHANG Hao, CHEN Wei, CHEN Yongquan. Effects of Folate Metabolism Inhibitors on the Proliferation and Differentiation of OP9 Mouse Stromal Cells[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201615036.

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Effects of Folate Metabolism Inhibitors on the Proliferation and Differentiation of OP9 Mouse Stromal Cells

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