Abstract: Objective: To explore the effect of Lycium barbarum polysaccharides (LBP) on cis-dichlorodiamineplatinum (II) (CDDP)-induced oxidative damage in mouse testis sertoli cells TM4 and its possible mechanism. Methods: The survival rate of the cells was evaluated by MTT assay. Superoxide dismutase (SOD) activity was examined by xanthine oxidase method. Malondialdehyde (MDA) content was measured by thiobarbituric acid assay. Glutathione (GSH) content was determined by nitrobenzoic acid method. Results: LBP at 5–100 mg/L could significantly enhance the survival rate of TM4 cells (P < 0.01), and at 20–100 mg/L could significantly inhibit the toxic effect of CDDP on TM4 cells (P < 0.01), and the best protective effect was observed as 50 mg/L. LBP at 50 mg/L could significantly reduce intracellular MDA content, increase SOD activity and GSH levels drop compared with CDDP control group (P < 0.01). Conclusion: CDDP-induced damage in TM4 cells could be remarkably reversed by LBP, which may be related with the strong antioxidant potential of LBP.

Key words: Lycium barbarum polysaccharides (LBP), cis-dichlorodiamineplatinum (Ⅱ), mouse testis sertoli cells TM4, oxidative damage