FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (10): 173-178.doi: 10.7506/spkx1002-6630-201810027

• Bioengineering • Previous Articles     Next Articles

Classification of Natural Plasmids in Lactobacillus brevis

SUN Daqing1,2, LI Hongfei1, YANG Jian2, SONG Dawei1,3   

  1. (1. National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. Mudanjiang Food and Biotechnology Innovation Institute, Heilongjiang Bayi Agricultural University, Mudanjiang 157000, China; 3. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China)
  • Online:2018-05-25 Published:2018-05-15

Abstract: This study aimed to establish a method for the classification of natural plasmids from Lactobacillus brevis. A total of 51 natural plasmids of L. brevis were accurately and efficiently divided into 6 family types and 11 subfamily types through the replication initiation protein (Rep) phylogenetic tree and genome colinearity analysis. A new type of replicon was found in the plasmids of family 4. The results showed that both the Rep phylogenetic tree and genome colinearity analysis were effective methods for the classification of natural plasmids in L. brevis. The classification results will provide a theoretical foundation for the scientific classification and reasonable application of L. brevis natural plasmids in the future.

Key words: Lactobacillus brevis, plasmid, replication initiation protein, genome colinearity, replicon

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