Isolation and Purification of Group B，Group E and DDMP-saponins from Soybean Hypocotyl

Abstract

Abstract: Total glycosides were extracted from soybean hypocotyl.The Group B saponin Ba and Bb,Group E saponin Bd and Be and DDMP saponin αg and βg were isolated and purified by C18 column chromatography and high-performance liquid chromatography (HPLC).The purity of isolate was detected with thin layer chromatogragh(TLC) and HPLC,the structure was confirmed with infrared(IR) spectra,mass spectra(MS)and nuclear magnetic resonance(NMR)spectra.

Key words: soybean, hypocotyl, soyasaponin, isolation

QUAN Ji-Shu, YIN Xue-Zhe, TIAN Zhong-Zhen-Shi, JIN Ze-Wu-Dao. Isolation and Purification of Group B，Group E and DDMP-saponins from Soybean Hypocotyl[J]. FOOD SCIENCE, 2003, 24(2): 107-109.

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Isolation and Purification of Group B，Group E and DDMP-saponins from Soybean Hypocotyl

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