Abstract: Selecting walnut seed, lotus seed, freezing-dried powder of Aloe, vegetable and fruit extracts(Euphorbia longan(Lour.)Steud., carrot, fructus jujubae, Tremella fucaiformis Berk.), pearl powder as main materials, reinforcing VE, VC, a kind of capsules offruit and vegetable capable of diminishing yellow-brown spots was investigated and prepared by using the .OH free radical scavengingability as evaluating index and screening model and exploiting orthogonal experiment design to optimize the formula. In order to inspectthe efficacy of screening model used above, the human experiments aiming at evaluating the diminishing yellow-brown spots ability ofproduct studied were carried out using the standard methods and experimental models recommended by "procedures and analyticalmethods for functional assessment on health food". The results suggested that the optimal formula product studied could remarkablydiminish the color density of yellow-brown spots(p<0.01),reduce the area of yellow-brown spots (p<0.01)and no new yellow-brown spots were generated after the products were administered orally to 50 healthy volunteers three times daily, three capsules pertime for thirty days. The volunteers under test had no obvious changes on the conventional and biochemical indexes of blood and no anyallergic reaction. Therefore, the optimal formula product studied has excellent free radical scavenging and yellow-brown spots dimin-ishing ability, and the model used in evaluating and screening formula of diminishing yellow-brown spots was successful and feasible.

Key words: capsules of vegetable and fruit, free radical scavenging, yellow-brown spots diminishing