Purification of Genistein and Daidzein from Pueraria Lobata(Wild.) Ohwi by Coordination Chromatography

Abstract

Abstract: To establish a protocol for the purification of genistein and daidzein from Pueraria lobata(Wild.)Ohwi by coordination chromatography. The extract of Pueraria lobata(Wild.) Ohwi was mixed with Nickel sulfate and then purified by dry column chromatography in gradient systems composed of chloroform and methanol. The elution order of genistein and daidzein was opposite comparing with ordinary chromatography method, and the purity of genistein and daidzein were greater than 95%.

Key words: Coordination Chromatography, Pueraria lobata(Wild.) Ohwi, Genistein: Daidzein, HPLC

PAN Jian, XU Tao. Purification of Genistein and Daidzein from Pueraria Lobata(Wild.) Ohwi by Coordination Chromatography[J]. FOOD SCIENCE, 2005, 26(9): 353-355.

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Purification of Genistein and Daidzein from Pueraria Lobata(Wild.) Ohwi by Coordination Chromatography

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