Abstract: Objective: The anti-fatigue effect of Yuanmi-D,a kind of import health food,was identified with a series of animal tests.Methods:The acute toxicitity LD50 test in mice,30 days feeding test ,wheel rotation test,swim test. Result: Yuanmi-D had no apparent acute toxicity on mouse, LD50 >10g/kgBW.During the 30 days of mouse feeding,all the mice in the treated group and the control group grew normally.The mouse bodyweight and the organ index had no apparent difference between the two groups.Wheel rotation time and swim time in the treated group lasted longer apparently than in the control group(p<0.05 and p<0.01) after first Yuanmi-Dfeeding.There was a trend of longer wheel rotation time and swim time in the treated group than in the control group after 20 days feeding(p>0.05).Conclusion: Yuanmi-D was safe in use, and there were some anti-fatigue function in Yuanmi-D on mice.

Key words: yuanmi-D, Anti-fatigue, wheel rotation test, swim test