Abstract: Aiming at cellulose and phytic acid,the anti-nutrition factors(ANF),existing in brown rice which make brown rice unsuitable for eating,the cellulase and phytase were directly put in brown rice in order to hydrolyze the cellulose and phytic acid in cortex of brown rice. The main factors including temperature,pH,consumption of enzyme solution,hydrolyzing time were respectively studied for each enzyme. Based on this,the orthogonal tests were conducted to optimize the reacting conditions. The results showed that the suitable conditions with cellulase were pH 3.5,10ml enzyme solution,at 55℃ for 90min,which for phytase were pH 2.5,15ml enzyme solution,at 55℃ for 150min. On the optimized conditions,phytic acid and cellulose in the cortex could be effectively decreased by using two enzymes together,meanwhile the hydrolyzing time was markedly shortened on the similar hydrolyzing results.

Key words: brown rice, anti-nutrition factor, cellulase, phytase